| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131318 |
| Chromosome: | chromosome 1 |
| Location: | 3536487 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022700 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTCAGCCACTGGCGTCGCCATTCACGT |
| Internal bar code: | CACGCCCATGAAATGTGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 97.8205 |
| LEAP-Seq n confirming: | 7226 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTAGGCCGCTGAGCATAA |
| Suggested primer 2: | GGAATGGCGTTCGTATGTCT |