| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131326 |
| Chromosome: | chromosome 8 |
| Location: | 4621768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383450 | FIST N domain protein; (1 of 3) PF08495 - FIST N domain (FIST) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTACGCCCGGGCCGGTGCAGGCGCATG |
| Internal bar code: | CTCAGACGAAACAACCGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGTGACCACCACCTTTC |
| Suggested primer 2: | CGTATCTTACGAAGGCGAGC |