| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131340 |
| Chromosome: | scaffold 24 |
| Location: | 97642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755847 | (1 of 1) PTHR11935//PTHR11935:SF104 - BETA LACTAMASE DOMAIN // METALLO-BETA-LACTAMASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGACAAACGAAATTGAGCAAAACGTG |
| Internal bar code: | CGCCAGGGCGCGCAAAATTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.5837 |
| LEAP-Seq n confirming: | 5741 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGCAGAAAGGGCTCAA |
| Suggested primer 2: | ATACTGCCGCCATGGTTTAG |