| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131360 |
| Chromosome: | chromosome 4 |
| Location: | 24884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213985 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGCTCGCCAGTCAGCCCGCCGGAGCCT |
| Internal bar code: | GGGCGCACTGAGAGTGGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 98.3811 |
| LEAP-Seq n confirming: | 5530 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACATTGTATCGGCGTG |
| Suggested primer 2: | GGCGTGTGTGTGTGTGTGTA |