| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131360 |
| Chromosome: | chromosome_9 |
| Location: | 3668149 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390615 | FAP12 | Triacylglycerol lipase and Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACAGCCTACATCGAGCGCGCGAACCGACC |
| Internal bar code: | ACGCGGACACGGGGACGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 90.9458 |
| LEAP-Seq n confirming: | 3827 |
| LEAP-Seq n nonconfirming: | 381 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAAGGGAGCAGACGTG |
| Suggested primer 2: | GTTGCAAGAATGCGAAGACA |