| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131365 |
| Chromosome: | chromosome 10 |
| Location: | 3689714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445952 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGGGCAGGTTGATTGGCATAGCCCTGT |
| Internal bar code: | GTATTATTGCTTGGGCATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 99.5873 |
| LEAP-Seq n confirming: | 18824 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAACCAACAGCAACCCAC |
| Suggested primer 2: | GTACTGTGCCGCATTAGGGT |