| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131372 |
| Chromosome: | chromosome_6 |
| Location: | 973860 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g255700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACGTTGATGCGGCGCAGCATCATGGCGTG |
| Internal bar code: | CGTGGCGTTGACGTCATCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 40.0922 |
| LEAP-Seq n confirming: | 174 |
| LEAP-Seq n nonconfirming: | 260 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCATGTCCCTTTCCTC |
| Suggested primer 2: | GACACAAAGCTGCAGCACAT |