Insertion junction: LMJ.RY0402.131372_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.131372
Chromosome:chromosome 12
Location:8833153
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCGCACGTGCCCTCCCTACCCCCTACTC
Internal bar code:TGTTGTTTGGTGCTTATTTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:792
LEAP-Seq percent confirming:98.0491
LEAP-Seq n confirming:2714
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGAACGAGGTGGGTATGA
Suggested primer 2:CACACACACACACACCTCCA