| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131386 |
| Chromosome: | chromosome 12 |
| Location: | 7556544 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555550 | (1 of 1) K05768 - gelsolin (GSN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCATCAAGGTGTATTACCGGCGTGACA |
| Internal bar code: | AGAGAGTTAAGGACCCGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 96.3646 |
| LEAP-Seq n confirming: | 1829 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTGAGGGTCGGTTCTG |
| Suggested primer 2: | TCGTGCCGTCTACTGACTTG |