| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131415 |
| Chromosome: | chromosome_1 |
| Location: | 6608891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g047300 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTGAGGAGTGGCGAAGCTGGGTTGGGGCT |
| Internal bar code: | GACGGGTCCTAACATGTGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.8923 |
| LEAP-Seq n confirming: | 8348 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTTTTAGGGTTTGGGG |
| Suggested primer 2: | TTTCAGTGCTGCTAACGGTG |