Insertion junction: LMJ.RY0402.131415_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.131415
Chromosome:chromosome 17
Location:2698396
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGTCACTAGACTTAACGCCACATCTTG
Internal bar code:TCTTTTTCCCGCTTTCTGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:99.5583
LEAP-Seq n confirming:5409
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTACTGCTTTGAGCTGGC
Suggested primer 2:CGGGCTTACTTGCTTGAGTC