| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131425 |
| Chromosome: | chromosome 6 |
| Location: | 5719539 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286850 | (1 of 1) 1.1.1.193 - 5-amino-6-(5-phosphoribosylamino)uracil reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATAGCAAGTCGGTCCTATCCGGGGGTGA |
| Internal bar code: | AGAAGGACCTAGATGCGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.8421 |
| LEAP-Seq n confirming: | 1265 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAGGGGAAATTAAGAGC |
| Suggested primer 2: | CTTTCCACCAACCCCAGTTA |