| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131452 |
| Chromosome: | chromosome 12 |
| Location: | 6261372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537100 | TSV,TSV2 | Valyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF5 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // VALYL-TRNA SYNTHETASE, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCTCTGCTGACCTCCCGTGTGTCGGAT |
| Internal bar code: | GTTGGCATCCGAAACAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.5772 |
| LEAP-Seq n confirming: | 1884 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGTACGTGCTCGCATT |
| Suggested primer 2: | GGGTCAAGACCACGACAACT |