| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131501 |
| Chromosome: | chromosome 8 |
| Location: | 4678732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383702 | UBC38 | (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGCAGGTACAGTTGCAGCGTTGCAGGG |
| Internal bar code: | GACAGGATCGCTACATGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 39 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTACGCCTTCACTTCTT |
| Suggested primer 2: | AACAGTACCCTTTCCCCCAC |