Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.131507 |
Chromosome: | chromosome 6 |
Location: | 3512695 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278107 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCGGATGCCGCCGGCACGAATGGCTTG |
Internal bar code: | TTTACGACTGGTCTTCGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 399 |
LEAP-Seq percent confirming: | 99.4477 |
LEAP-Seq n confirming: | 4862 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCCCATAACTAGCCCTAT |
Suggested primer 2: | AGGTGAGTCCATTTGTTGGC |