Insertion junction: LMJ.RY0402.131511_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.131511
Chromosome:chromosome 1
Location:7810918
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g055966 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCCGGAAGTGCGACAGCGCGTGGTA
Internal bar code:TCTGTAACCGGGTGGCTGAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:99.7714
LEAP-Seq n confirming:5237
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGCAGCCAGGAGACAAT
Suggested primer 2:TTTAGATCACACACGCAGCC