| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131536 |
| Chromosome: | chromosome 17 |
| Location: | 1597817 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707750 | (1 of 1) PTHR31040//PTHR31040:SF1 - FAMILY NOT NAMED // NURIM | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTAGCATGTCCGGCTACCTGCCGAGC |
| Internal bar code: | CAGGGACGGGAAAAGTGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 99.21 |
| LEAP-Seq n confirming: | 9167 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTATTCGTCACCTGTGC |
| Suggested primer 2: | CCCTACATGTATCCGCGTCT |