| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131538 |
| Chromosome: | chromosome 9 |
| Location: | 4874873 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397734 | (1 of 2) K07424 - cytochrome P450, family 3, subfamily A (CYP3A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATTTAAGAAGTGGCGTAGGGCGGGGTC |
| Internal bar code: | TCGGCCCGTTGTAATGTGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.4979 |
| LEAP-Seq n confirming: | 8917 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGGACTACAGAACGGGT |
| Suggested primer 2: | CAATGACACAGGTGTTTGGC |