| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131541 |
| Chromosome: | scaffold 24 |
| Location: | 96704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCATACGGTCCAAACAACCGCAAGCTTG |
| Internal bar code: | CCACGGCGCTCTTGTGTCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 98.9467 |
| LEAP-Seq n confirming: | 6294 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGGTAATCAAAGGGA |
| Suggested primer 2: | TTGAGCCCTTTCTGCAAGTT |