| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131548 |
| Chromosome: | chromosome 11 |
| Location: | 3687823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482650 | EZY3 | Early zygote expressed protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGCAACTGGTGTGCGTATGCCAAAGGC |
| Internal bar code: | GGGCCAAGAAGCCGATTGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.5626 |
| LEAP-Seq n confirming: | 8195 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTATGTGCGGTAGTCAT |
| Suggested primer 2: | AACTGAGTGTGGGTTCGGTC |