| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131582 |
| Chromosome: | chromosome 12 |
| Location: | 2437549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507000 | FFT6 | putative fructan fructosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGCGGTGGTGATGGCGGCGGCCGGCAG |
| Internal bar code: | ACGGGCGTGACATTACGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 43.1363 |
| LEAP-Seq n confirming: | 15627 |
| LEAP-Seq n nonconfirming: | 20600 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATTCGCTTATTCACGCA |
| Suggested primer 2: | TGATGATGAGGAGGAGGAGG |