Insertion junction: LMJ.RY0402.131598_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.131598
Chromosome:chromosome 10
Location:3044052
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g441300 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCATCCCACCAGTGGGATAAACAAGGAC
Internal bar code:ACGATCCAAGGTCAGTGCTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:99.5174
LEAP-Seq n confirming:1031
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTGTGTTCGTCATGGTC
Suggested primer 2:GTGAGCTACGGGTTACGAGC