| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131600 |
| Chromosome: | chromosome 12 |
| Location: | 5633864 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532200 | AOA2 | Putative alcohol O-acetyltransferase; (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTACAACCGCGCTCACCGCGTATGTGAA |
| Internal bar code: | CGCACTTTATACGCTCCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.7368 |
| LEAP-Seq n confirming: | 2653 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGACTGGAAGGCTGTGCT |
| Suggested primer 2: | TCATGGCACTGCAGCTTAAC |