| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131608 |
| Chromosome: | chromosome_3 |
| Location: | 3973871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g171800 | FAP123 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGGTACCGGGGCCTCGACCCAGCGTTGT |
| Internal bar code: | ACAAACGGCGGTTACCATCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.2527 |
| LEAP-Seq n confirming: | 8235 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATAGCTATGCACGCAA |
| Suggested primer 2: | ACACGAAACCGAATGACACA |