Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.131643 |
Chromosome: | chromosome_1 |
Location: | 6276441 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g044850 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGAGGCAGCGGACGTACACGGCCTGCGC |
Internal bar code: | GCTGCTTCTCAGGGGCCCCCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 241 |
LEAP-Seq percent confirming: | 98.9726 |
LEAP-Seq n confirming: | 289 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGGCACTTTGAGCATTTG |
Suggested primer 2: | AACCCTGGCGGTCTACTTCT |