| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131664 |
| Chromosome: | chromosome 10 |
| Location: | 1045603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425200 | (1 of 1) 1.8.3.5 - Prenylcysteine oxidase / Prenylcysteine lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGCAGGGCCGGGCAAGCAGGGTTGCG |
| Internal bar code: | GCAGTGCACATTTTGGCGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 99.6751 |
| LEAP-Seq n confirming: | 24546 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTGCAAGCATATCAAC |
| Suggested primer 2: | TATATGTTTGCCGGTCCGAT |