| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131676 |
| Chromosome: | scaffold_19 |
| Location: | 47601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre19.g750447 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTACAGTACCAAACAGCGGGTGCGGGGGC |
| Internal bar code: | TGCACGTAGAACAGGCAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 99.7321 |
| LEAP-Seq n confirming: | 1117 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTCTGGGTTGTTCCACG |
| Suggested primer 2: | GTTTCATGTGCCATGTCCAG |