Insertion junction: LMJ.RY0402.131691_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.131691
Chromosome:chromosome 6
Location:3512483
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278107 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCTTTAAAGCTAGAAAGGGCAATAGCC
Internal bar code:AACTGTACGTACGCGCCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:99.6678
LEAP-Seq n confirming:2400
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATAGAAGCGTTCGGGAC
Suggested primer 2:AGGTGAGTCCATTTGTTGGC