Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.131691 |
Chromosome: | chromosome 6 |
Location: | 3512483 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278107 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTTTAAAGCTAGAAAGGGCAATAGCC |
Internal bar code: | AACTGTACGTACGCGCCTCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 396 |
LEAP-Seq percent confirming: | 99.6678 |
LEAP-Seq n confirming: | 2400 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGATAGAAGCGTTCGGGAC |
Suggested primer 2: | AGGTGAGTCCATTTGTTGGC |