| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131717 |
| Chromosome: | chromosome 1 |
| Location: | 324803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001950 | Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTGGGAGTACCAGGGGGGGGGGAGGT |
| Internal bar code: | TTCGGCCTCTAGCCCGGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 93.007 |
| LEAP-Seq n confirming: | 665 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATACGGCTTGGACGAACC |
| Suggested primer 2: | AGTGGTGTGGGAGAGGTTTG |