| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131717 |
| Chromosome: | chromosome 1 |
| Location: | 7427172 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053300 | AIH1 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTCAAACAAGCAGGAGTTATCCAGTA |
| Internal bar code: | GGGGTTTTAGGGATGTAATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 99.0097 |
| LEAP-Seq n confirming: | 8598 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTTGCCATGTTGTGTG |
| Suggested primer 2: | TGCCATGTCCATATGCTTGT |