| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131743 |
| Chromosome: | chromosome 12 |
| Location: | 1755585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512450 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGCGCCATGGACGGCCCCGTCAGCAG |
| Internal bar code: | TTGGTGGGATGTTCGTAATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 18.9189 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCCGACATGTAAGACT |
| Suggested primer 2: | GTGGTTCTTCTTCGTCCCTG |