| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131761 |
| Chromosome: | chromosome 1 |
| Location: | 4923529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034100 | (1 of 2) K01408 - insulysin (IDE, ide) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGCGGCGGTCAAGCGCGCGCGAAGCA |
| Internal bar code: | GCATCCGTTCACTGCTCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.2269 |
| LEAP-Seq n confirming: | 7958 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCTTGCAAACAAGTCC |
| Suggested primer 2: | CACATTCTTGTCGAGGCAGA |