| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131768 |
| Chromosome: | chromosome 2 |
| Location: | 4865536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103250 | MRP5 | (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCACGGCGGCGTGTGATGGCCCGCTGCA |
| Internal bar code: | GGTTATGAGGCGCACTGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 97.4547 |
| LEAP-Seq n confirming: | 4518 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTATGTCCGCCAAG |
| Suggested primer 2: | GTGGCAGGGGGAGTTTTACT |