| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131802 |
| Chromosome: | chromosome 1 |
| Location: | 2333963 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012750 | KCN7 | (1 of 4) IPR000595//IPR002110//IPR005821//IPR018490//IPR020683 - Cyclic nucleotide-binding domain // Ankyrin repeat // Ion transport domain // Cyclic nucleotide-binding-like // Ankyrin repeat-containing domain; Voltage-gated potassium channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATCCTGTTGTGCTGTGTTTCTGGTGCG |
| Internal bar code: | CAAGCCGAATATGACTGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 88.0478 |
| LEAP-Seq n confirming: | 2210 |
| LEAP-Seq n nonconfirming: | 300 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCATATTGGGTGCTTTTC |
| Suggested primer 2: | ACCCATGTAGTCCTTGGCAG |