| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131835 |
| Chromosome: | chromosome 12 |
| Location: | 9345890 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540650 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATACAGCCTGCACGATATGCAGACAAT |
| Internal bar code: | AGGGGGTTAGTTCAAGGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 99.7113 |
| LEAP-Seq n confirming: | 4144 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCACTGCGAGTCAGGAAC |
| Suggested primer 2: | CAGGGGATTAAGCATCCAGA |