| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131876 |
| Chromosome: | chromosome 16 |
| Location: | 6713330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672161 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGTGCATCGGGCGCGGGGCGCAGTGGG |
| Internal bar code: | GCAAGGTATTCCGTGAGCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 98.3122 |
| LEAP-Seq n confirming: | 233 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGAGCAGTCAACTCTG |
| Suggested primer 2: | ATGGCAACGGTAAGGCAAC |