| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131910 |
| Chromosome: | chromosome 16 |
| Location: | 3163756 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666050 | CPLD49,SCD1 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP); Cytochrome b6f biogenesis protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACTGAATTGCGCATGCGAGTGCACGTG |
| Internal bar code: | CGCATTTGATTTATGCTTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 86.8313 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTCACCTCGCAGTCAG |
| Suggested primer 2: | CGGTAAGGCAGCCTGAGTAG |