| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131921 |
| Chromosome: | chromosome 7 |
| Location: | 5188922 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348550 | TGL13 | (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTACCTGGCCGGCACCATGTCCTACTAT |
| Internal bar code: | GCGCACGGTTCGTGCCCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 95.2884 |
| LEAP-Seq n confirming: | 1173 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGGTGCAGGTTTGTAA |
| Suggested primer 2: | TGGCGGGTACAACATCACTA |