| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131928 |
| Chromosome: | chromosome 16 |
| Location: | 586777 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692300 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGCTGAGGCCGCCCACGCCCATTGCG |
| Internal bar code: | GAGTCAGTGGGATCGTTGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.5283 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAACGAATGGTGACAAC |
| Suggested primer 2: | GAGTTCGTGACCTACGCCTC |