| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131949 |
| Chromosome: | chromosome 9 |
| Location: | 1831278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395950 | AOX1 | Alternative oxidase, mitochondrial; (1 of 2) PTHR31803//PTHR31803:SF3 - FAMILY NOT NAMED // ALTERNATIVE OXIDASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGTAACGCATGCTGATGCCCCATGTA |
| Internal bar code: | GTTTGACTGATCGGCGGCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 99.4487 |
| LEAP-Seq n confirming: | 9380 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTTCCCTCACCACACAA |
| Suggested primer 2: | AGGGCAGAAGGCACTCAGTA |