| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131964 |
| Chromosome: | chromosome 9 |
| Location: | 3836370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391838 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGCTACAAGGCCTGCGTTGGTGGTTG |
| Internal bar code: | CGCGAACCGAAAAAACTCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 99.8117 |
| LEAP-Seq n confirming: | 10599 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGCCGCTTCCTAGTGAT |
| Suggested primer 2: | GGAATCGATGGCAGATATGG |