| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131969 |
| Chromosome: | chromosome_6 |
| Location: | 1200028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257600 | FA1 | Flagellar Autonomy Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCATTGCGCCACCGCCGGCAGCAGATCAT |
| Internal bar code: | TCAGAGGCCGGATGTTAAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 92.0794 |
| LEAP-Seq n confirming: | 8533 |
| LEAP-Seq n nonconfirming: | 734 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCATTGACCATCAGTC |
| Suggested primer 2: | TACATTGACCCGCTCACATC |