| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131970 |
| Chromosome: | chromosome 7 |
| Location: | 2979438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATCAGGCAGGTCCGTAAGTTCACACAT |
| Internal bar code: | CCTTGTTTTGCTCGCACCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1432 |
| LEAP-Seq percent confirming: | 94.8932 |
| LEAP-Seq n confirming: | 3066 |
| LEAP-Seq n nonconfirming: | 165 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAACTGCCTTACCGTGG |
| Suggested primer 2: | GACTCTTCGATCAGAACGGC |