| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131994 |
| Chromosome: | chromosome 3 |
| Location: | 8047271 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201350 | (1 of 100) IPR001810 - F-box domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCGAAGGGTCGGCCGACACGCTGCAT |
| Internal bar code: | CAGCGGCGATGCATGAGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 99.7171 |
| LEAP-Seq n confirming: | 5993 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGGCGGACTAGTGATG |
| Suggested primer 2: | CTTGCTTGAGAAGTCCCCTG |