| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131999 |
| Chromosome: | chromosome 3 |
| Location: | 9125400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211745 | (1 of 1) K00666 - fatty-acyl-CoA synthase [EC:6.2.1.-] (K00666) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTTATGCCGAAAACAAGCACACAGACA |
| Internal bar code: | CCCTTGGCGGTGACACCTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 24 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTACGAAGTGGGGGTAGC |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |