Insertion junction: LMJ.RY0402.132001_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.132001
Chromosome:chromosome 4
Location:3299764
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g226950 (1 of 1) K06693 - 26S proteasome non-ATPase regulatory subunit 9 (PSMD9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGCGCCGTTGCCAGCAGCCGCACCCAG
Internal bar code:ACCCGGGTCCCTGTGGTTGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:240
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACACGTACCGCGTACACA
Suggested primer 2:CAAACGCATACAGCTCTCCA