Insertion junction: LMJ.RY0402.132007_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132007
Chromosome:chromosome 14
Location:3655577
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g631400 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCAGCAACCCAGAATTCCACCATCTCA
Internal bar code:CGCGAAGGCAGAGAGCGCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:56.1169
LEAP-Seq n confirming:3110
LEAP-Seq n nonconfirming:2432
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTACTGCTGGGAAGAGTCGC
Suggested primer 2:GGTGTTAGCAAGTCACCCGT