| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132009 |
| Chromosome: | chromosome 12 |
| Location: | 1276763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489200 | (1 of 1) PTHR10794//PTHR10794:SF28 - ABHYDROLASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCGCTGCCGTGACTCCTTCATTCAGC |
| Internal bar code: | GGGCTCTCCCCTATGATAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.3493 |
| LEAP-Seq n confirming: | 3970 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGTGGGTGGCTATTTG |
| Suggested primer 2: | AGGAACGTGAGGTGATACGG |