| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132022 |
| Chromosome: | chromosome 12 |
| Location: | 8269143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550200 | SMM44 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGCGTGGTGCCAGGCCGTCTCCCCGA |
| Internal bar code: | TTCCGCGCGCTGGACTAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.25 |
| LEAP-Seq n confirming: | 5690 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTCTTCTGGAAGGTGCG |
| Suggested primer 2: | AATTGCGGTTGCTTGTTACC |