| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132030 |
| Chromosome: | chromosome 16 |
| Location: | 415139 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATAGTAACCGCCGCCACCCCCGTAAT |
| Internal bar code: | AAGGTCTATTATGCAAGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 98.9619 |
| LEAP-Seq n confirming: | 858 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCGGTAACATTGTGGA |
| Suggested primer 2: | TACTCGAGTCCGAAGCACCT |